Insertion junction: CLIP2.034439_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.034439
Chromosome:chromosome 4
Location:3854535
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g230144 SPT1 Component of serine palmitoyltransferase; (1 of 1) PTHR13693//PTHR13693:SF3 - CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE // LD36009P intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGTACCATCGTGCACCTAGACCCCGCACGATGGGCTGTGTCCCACTCT
Internal bar code:CGTCCATTCCCCTAGAGCTTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:778
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CAACCTCCCTTCCAATCCCC
Suggested primer 2:CAGCCCCCATTCTTGGTCAT