| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.034455 |
| Chromosome: | chromosome 12 |
| Location: | 8408062 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550300 | (1 of 1) PTHR24361//PTHR24361:SF276 - MITOGEN-ACTIVATED KINASE KINASE KINASE // PROTEIN KINASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCACGGGCAGCAGCACCAGCACCAGCACCAGCACCAGCAGCTGACGCA |
| Internal bar code: | TCCAGTGGTGGGGTAGGTCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2351 |
| LEAP-Seq percent confirming: | 98.5714 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGTGAGTGTACGGCAGC |
| Suggested primer 2: | GGGTTTCAGTGGTTTCGTGC |