Insertion junction: CLIP2.034471_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.034471
Chromosome:chromosome 11
Location:4060971
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g480800 (1 of 1) K14773 - U3 small nucleolar RNA-associated protein 23 (UTP23) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGCGGGCGACACCTCACTCGCCACGGCTTCGTCCGCCCCCACTCCTCC
Internal bar code:GGCTTGGCGACGCTAGTAGCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:603
LEAP-Seq percent confirming:86.6667
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTTCCTCCCTTCTGCTCC
Suggested primer 2:GCACACCATGACACACACAC