| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.034476 |
| Chromosome: | chromosome 9 |
| Location: | 3613582 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396846 | (1 of 2) PTHR22957//PTHR22957:SF148 - TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN // RE26521P | CDS | |
| lncRNA_TCONS_00135978 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTGCCAGCAGCGCGGCAGCTCCTTAGCCGGGGTCCTGCGTTCGGGGAA |
| Internal bar code: | TTATCGTCTTGCTCGTGGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5506 |
| LEAP-Seq percent confirming: | 83.5821 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCACCACTCGCACATTG |
| Suggested primer 2: | GTATCCCCCGTAGCTGAAGC |