| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.034480 |
| Chromosome: | chromosome 12 |
| Location: | 6532777 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538000 | EZY5 | Early zygote expressed protein; (1 of 26) IPR001202 - WW domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCCGTCCTGGTTCATCTTGCCGCGCTGCTTCTGCGGCTTGTTCTTGC |
| Internal bar code: | AGAGAAGCGGTAAGCTAGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3524 |
| LEAP-Seq percent confirming: | 95.6522 |
| LEAP-Seq n confirming: | 154 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 161 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCATCATTTACCTGCAGG |
| Suggested primer 2: | CCATGGTGCTGTGTTTGGTG |