Insertion junction: CLIP2.034484_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.034484
Chromosome:chromosome 7
Location:4201904
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g342000 (1 of 1) PF00651//PF12847 - BTB/POZ domain (BTB) // Methyltransferase domain (Methyltransf_18) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTACGTTTCTACCCACCCTGCACCCGACCCACATGCCGACCCGCGCCGC
Internal bar code:GGATTAGTGGAGCGCGTGCTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1014
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGTGCGAGAGACGTCAAA
Suggested primer 2:CTCTCCAACACAGCGCAAAC