| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.034494 |
| Chromosome: | chromosome 12 |
| Location: | 1984487 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g511050 | RSP16 | Radial Spoke Protein 16; (1 of 1) K09519 - DnaJ homolog subfamily B member 13 (DNAJB13) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCATCGGGGTGGGTGCACACGCACGCACGCCCCGGGCCGCGCGACCCT |
| Internal bar code: | TGTACGTGGGAACCATTTCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3080 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAATATGTCCAGCGCTTG |
| Suggested primer 2: | GCTCTCCTCTGGGTTGAACC |