| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.034494 |
| Chromosome: | chromosome 12 |
| Location: | 1984493 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g511050 | RSP16 | Radial Spoke Protein 16; (1 of 1) K09519 - DnaJ homolog subfamily B member 13 (DNAJB13) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTAGGCAATGCACTGTGCCACGAACCACGCGGATTCATGGTGGCGGC |
| Internal bar code: | CTATGATCTTTTTAAATTTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4127 |
| LEAP-Seq percent confirming: | 99.115 |
| LEAP-Seq n confirming: | 112 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 113 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTCCTCTGGGTTGAACC |
| Suggested primer 2: | GCCAATATGTCCAGCGCTTG |