Insertion junction: CLIP2.034499_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.034499
Chromosome:chromosome 2
Location:1526784
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g084300 COQ5,UMM2,COQ5C,MENG (1 of 1) K03183 - demethylmenaquinone methyltransferase / 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase (ubiE); Phylloquinone biosynthesis methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGCCTCCCACGGTTTCATCGCAACCCAAGAGCTGTAAAATTGTAAAC
Internal bar code:AATGTGGCGGGACCATAATGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:987
LEAP-Seq percent confirming:62.2642
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCACACTGCATCACAAAA
Suggested primer 2:AAACGCTGGCGGTACTTGTA