| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.034508 |
| Chromosome: | chromosome 2 |
| Location: | 6770476 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387838 | (1 of 3) PF03647 - Transmembrane proteins 14C (Tmemb_14) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGATGCCCACCTGGATGGTCCAGGCGGTGGCCCCGTACGCCGCACCAA |
| Internal bar code: | ACTCTTAAGTACTAATGTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3472 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTTCAAGCGACCCCATA |
| Suggested primer 2: | GCGCACAACTCTTTCCGTTT |