Insertion junction: CLIP2.034531_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.034531
Chromosome:chromosome 3
Location:6567795
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g194500 (1 of 2) IPR000719//IPR011009//IPR013221 - Protein kinase domain // Protein kinase-like domain // Mur ligase, central intron
Cre03.g194517 (1 of 34) IPR001878 - Zinc finger, CCHC-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTCAACACTCCCCCGCGGGCAGAACTCCATACTCAAATTACGTATCGC
Internal bar code:GATTAATGTACTTCCATATCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3787
LEAP-Seq percent confirming:6.77966
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:55
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTATGATGCCGGTAGTGC
Suggested primer 2:GCAGATCCACCTCCATCTGG