Insertion junction: CLIP2.034563_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.034563
Chromosome:chromosome 10
Location:635036
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g421900 SRR27 Scavenger receptor cysteine rich (SRCR) protein; (1 of 5) 1.4.3.13 - Protein-lysine 6-oxidase / Lysyl oxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGACCCCGCAACATACAGGCAACAACGTGTCCATACATGTTTGCGCGCC
Internal bar code:CGTAATACCGGGGTGAGGTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2689
LEAP-Seq percent confirming:40.7407
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:TCCAACACGGCTGGTAACTC
Suggested primer 2:TGTCCAGGTACGCTAGAGCT