| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.034584 |
| Chromosome: | chromosome 1 |
| Location: | 2494724 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g800047 | (1 of 14) K00472 - prolyl 4-hydroxylase (E1.14.11.2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCCCGCGGCGCTTAAGAGAAGCCCTTCGTGCTGGCACGGCTCACCTGT |
| Internal bar code: | ACAACTAGGTATTATGAGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 796 |
| LEAP-Seq percent confirming: | 40.4762 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGGCGCGTTCATAATTC |
| Suggested primer 2: | GAGCGTGCAACAAACGAGTT |