Insertion junction: CLIP2.034585_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.034585
Chromosome:chromosome 10
Location:2387270
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g434950 (1 of 1) PF05010 - Transforming acidic coiled-coil-containing protein (TACC) (TACC) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCTGGTCTATGTCGACCTGCGCTGTGACTGACCTTTGCATCCACGGGT
Internal bar code:TGGGCGATTAGAACTGACGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:3523
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:71
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:71

Suggested primers for confirmation by PCR

Suggested primer 1:AACAAACATCCAAACGCCCG
Suggested primer 2:ATTACGCTGCTGCTCCTTGT