| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.034587 |
| Chromosome: | chromosome 12 |
| Location: | 9874223 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543902 | (1 of 1) 2.7.1.16 - Ribulokinase / L-ribulokinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTCCAAGCTTATCGCGGATAAGTCTGACTACCTGGGTCACGGCAGCC |
| Internal bar code: | AAGACCTGACTTTCGTTGATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1652 |
| LEAP-Seq percent confirming: | 56.6667 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCGTGCCTTCCTCACTT |
| Suggested primer 2: | CGCGACACATTTAAGGCTCG |