Insertion junction: CLIP2.034592_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.034592
Chromosome:chromosome 11
Location:3421628
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g477200 IFR1 Putative 2'-hydroxyisoflavone reductase; (1 of 19) PF01370 - NAD dependent epimerase/dehydratase family (Epimerase) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCAGTTCGCTGACGCCTAGTTAGGCGTCTCAGTCTTACGCCAGTCCTT
Internal bar code:GGGCTATCCCAGGGTATCATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2826
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GTACTCAGCCAAACCGACCA
Suggested primer 2:CACCAGCTCACAAACAGCAC