Insertion junction: CLIP2.034596_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.034596
Chromosome:chromosome 9
Location:703622
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g404503 (1 of 3) K16489 - protein SFI1 (SFI1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCAAAGTAGGGGGAGGAGCACGTAGCTGGGGAAGCCCCTGCGTGAAGC
Internal bar code:ATTTAATACGGGGGCTCAAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2390
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:TGCATGCTTCATAGGGTGCA
Suggested primer 2:CTACTGCAACCTCGACAGCA