Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.034622 |
Chromosome: | chromosome 5 |
Location: | 2635505 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre05.g240050 | (1 of 1) PF13813 - Membrane bound O-acyl transferase family (MBOAT_2) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTCATCGCCGGCTCGCCGTCGCCCCGACGAATGCGCATGCGACTGCTC |
Internal bar code: | ACGTTACTTAGCGGGCGCAGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1998 |
LEAP-Seq percent confirming: | 90.3226 |
LEAP-Seq n confirming: | 28 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCCCTTGAATCCCGACTTC |
Suggested primer 2: | TGCCTACACGGTGCAATTCT |