| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.034622 |
| Chromosome: | chromosome 5 |
| Location: | 2635509 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240050 | (1 of 1) PF13813 - Membrane bound O-acyl transferase family (MBOAT_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAAAGCTTCCAGCAGGCGCGGTAGGCTCGCAAGCAGCCACAGCAGCAGC |
| Internal bar code: | ACGTTACTTAGCGGGCGCAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 101 |
| LEAP-Seq percent confirming: | 36.3636 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCTACACGGTGCAATTCT |
| Suggested primer 2: | CCCCCTTGAATCCCGACTTC |