| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.034624 |
| Chromosome: | chromosome 17 |
| Location: | 3914148 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g727850 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAAATTAGCGTCTCAGCAGGGTCCGGTGCTTCCACGTGCAAACCGCCAG |
| Internal bar code: | TATTGTGACCATCGTCACTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1618 |
| LEAP-Seq percent confirming: | 55.0 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGATGACCCACAGCAGAA |
| Suggested primer 2: | TGAAGTGGCAGTAGCTGTCG |