Insertion junction: CLIP2.034631_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.034631
Chromosome:chromosome 13
Location:2209160
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g578150 RRAA1,RRA1 Regulator of ribonuclease activity A; (1 of 1) 4.1.1.3 - Oxaloacetate decarboxylase / Oxaloacetate carboxy-lyase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTCCTGCTGCGCGCCTCCTGCGCTCCATGGCTCGCTCCCCCCAACCTC
Internal bar code:AACAAGTGTGTGGTATTTTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3083
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:57
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:CACGCCCAGCATTTTGTCAA
Suggested primer 2:CGAGTAGTGTGTCCGTGTGT