| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.034644 |
| Chromosome: | chromosome 3 |
| Location: | 1070535 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g148450 | TEH1 | Acetyl-CoA thioesterase/hydrolase; (1 of 1) 3.1.2.1 - Acetyl-CoA hydrolase / Acetyl-CoA deacylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTATCCGGCCTATAGCACGTCGTCTTGTCCACGTGCGCACGACACCG |
| Internal bar code: | TAATTGCTCTGTAGAACGTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3470 |
| LEAP-Seq percent confirming: | 68.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACCAACGAAAACGAGCGG |
| Suggested primer 2: | GGGGGAATTGGAGTTGCAGA |