Insertion junction: CLIP2.034645_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.034645
Chromosome:chromosome 1
Location:4583470
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g031550 CSB6 (1 of 12) IPR004843//IPR029052 - Calcineurin-like phosphoesterase domain, apaH type // Metallo-dependent phosphatase-like; Probable transposon-derived protein of Chlamydomonas-Specific family B intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGGTGGGGTTGGCTCAAGGTGGGGTGGGGTTGGGGGGCGGCCAGGGGA
Internal bar code:CTTAAAATCGACCGACGTTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:942
LEAP-Seq percent confirming:91.6667
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CATTCCTTTGCTTCGCCGAG
Suggested primer 2:CCTAGAGGCTACCCACCCTT