| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.034650 |
| Chromosome: | chromosome 3 |
| Location: | 3237529 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165350 | (1 of 3) 1.1.1.30 - 3-hydroxybutyrate dehydrogenase / D-beta-hydroxybutyrate dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTCCGTTTGCGTCGGCGCGCCGCATACCGTACGGCATCACCCACCAT |
| Internal bar code: | TCTGGGGGTTATTTAAGGTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3669 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTACCCGCACTCACACTG |
| Suggested primer 2: | ACAGTGTGCAGGAGCTAACC |