Insertion junction: CLIP2.034668_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.034668
Chromosome:chromosome 14
Location:2317109
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g622850 RAD51A,RAD51 (1 of 1) K04482 - DNA repair protein RAD51 (RAD51); RAS associated with diabetes protein 51 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAAGATCATCGCCAGCCCCTCGCTGCCGGAGCGCGAGGCCAACTTCGC
Internal bar code:CTAGTATCCAATTGTTGCAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1387
LEAP-Seq percent confirming:95.2381
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:GACTCGACACCTGCACTTCA
Suggested primer 2:CACCTTGCTCCAAACCATGC