Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.034673 |
Chromosome: | chromosome 10 |
Location: | 3861452 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g446100 | TRX6,TRXY,TRXY1 | Thioredoxin y, chloroplastic; (1 of 1) PTHR10438:SF280 - THIOREDOXIN Y1, CHLOROPLASTIC-RELATED | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATAGGTTATGGACTTTGTGCATGCACAGACGTTGCAGCGCAGCCTCAC |
Internal bar code: | CGCGGTCACGCATAAAATACGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3900 |
LEAP-Seq percent confirming: | 92.5926 |
LEAP-Seq n confirming: | 25 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGGTGGACTTCTACGCAACC |
Suggested primer 2: | GTCATGTGCATGCACCCATC |