Insertion junction: CLIP2.034673_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.034673
Chromosome:chromosome 10
Location:3861452
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g446100 TRX6,TRXY,TRXY1 Thioredoxin y, chloroplastic; (1 of 1) PTHR10438:SF280 - THIOREDOXIN Y1, CHLOROPLASTIC-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATAGGTTATGGACTTTGTGCATGCACAGACGTTGCAGCGCAGCCTCAC
Internal bar code:CGCGGTCACGCATAAAATACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3900
LEAP-Seq percent confirming:92.5926
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTGGACTTCTACGCAACC
Suggested primer 2:GTCATGTGCATGCACCCATC