| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.034694 |
| Chromosome: | chromosome 16 |
| Location: | 5025579 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686050 | (1 of 1) PF05277 - Protein of unknown function (DUF726) (DUF726) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTATTTACTGAAGAAGTGAGGGGGGGGGGAGGAAGAGCCACTCATGCGG |
| Internal bar code: | GTCGCTAAAGGACAAGCAAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1017 |
| LEAP-Seq percent confirming: | 63.0435 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCTTCTCACCCCCTCCTC |
| Suggested primer 2: | ATGTTCTCAATGCCCTGGGG |