| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.034699 |
| Chromosome: | chromosome 2 |
| Location: | 2165305 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g089350 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGACGTGTGCCGGGGGTGCTGTGAGTGCCGGCCTTGGATATCGTGTTG |
| Internal bar code: | AGAAGCACTGCGTTCGGTTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 612 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGCAGCAACTTGAACCC |
| Suggested primer 2: | ATGGGTTTGGTGTGGTTGGT |