| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.034701 |
| Chromosome: | chromosome 10 |
| Location: | 5523078 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458100 | YEE1 | Membrane protein; (1 of 3) K07112 - uncharacterized protein (K07112) | outside_mRNA |
| Cre10.g458150 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTGCTCATGCCTGTCACGCTAGCCGGCAGCTTGGGGGACGTTGGCGAC |
| Internal bar code: | CTACTCTAGCACCAGGAATTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1518 |
| LEAP-Seq percent confirming: | 53.3333 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTTTGACCGCTAGCACG |
| Suggested primer 2: | GCGCTGTTGAACTTGAGCAA |