Insertion junction: CLIP2.034704_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.034704
Chromosome:chromosome 9
Location:2677033
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g391900 TRXH1,TRXH,TRXh1,Chh1,TRX5 (1 of 9) K03671 - thioredoxin 1 (trxA); Thioredoxin h1, cytosolic intron
Cre09.g801021 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGACATGGCACATGACACCGCTGCGGGCTCGTCGGGGAACACGCTCTAA
Internal bar code:TGGAGAAATGCATTGATCTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:526
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CAACCCTTTCCTCGCTTCCT
Suggested primer 2:GGTCACTGTCACTGTCCCTG