| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.034726 |
| Chromosome: | chromosome 14 |
| Location: | 4148932 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g634100 | (1 of 7) IPR010995 - DNA repair Rad51/transcription factor NusA, alpha-helical | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGAATGGCTTCACCAGCCATTTACAGCAAATTATTCTTACTATGTAC |
| Internal bar code: | GACCTCTTTCAAAAAGCACAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6262 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAATTTCTTAGCGGCGCTG |
| Suggested primer 2: | CCAATCTGCGCATGCTCTTC |