| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.034729 |
| Chromosome: | chromosome 3 |
| Location: | 1040213 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g800291 | (1 of 9) 3.2.1.78 - Mannan endo-1,4-beta-mannosidase / Endo-1,4-mannanase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCGTGGCTAAACTCAACACAGAGCACGCTGCGGCGGACGGTCGTGAG |
| Internal bar code: | TCGCTACACGTTACCCCCCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1060 |
| LEAP-Seq percent confirming: | 95.6522 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCAGCAAGTCAAACACG |
| Suggested primer 2: | TTACACGGTCCTTGTCCACG |