Insertion junction: CLIP2.034730_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.034730
Chromosome:chromosome 4
Location:3521805
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g228150 FAE1 (1 of 1) PF02797//PF08392//PF08541 - Chalcone and stilbene synthases, C-terminal domain (Chal_sti_synt_C) // FAE1/Type III polyketide synthase-like protein (FAE1_CUT1_RppA) // 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal (ACP_syn_III_C); Putative beta-ketoacyl-coa synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCACGCACACCCGCCCCCCCTCCCCGCCCCCCAGGTCCTGCGTCACTCA
Internal bar code:AAAGGCTCATGGCTACTTTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:647
LEAP-Seq percent confirming:20.8333
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:GCATGCCCATACTTTGCGAG
Suggested primer 2:CCACTCACCTTGGGCGTAAT