Insertion junction: CLIP2.034732_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.034732
Chromosome:chromosome 3
Location:8364834
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g800397 (1 of 1) K18735 - protein SMG9 (SMG9) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCTGTCTTTGCGTAACGTTTTCCTTGTGCTCTTTAACACACCGATGTG
Internal bar code:TGCCGCTCACAACTGTTGAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:87
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTACTGCCCTTCGTAGG
Suggested primer 2:CGCGCTAACATGTTCCTTCG