| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.034742 |
| Chromosome: | chromosome 9 |
| Location: | 2592869 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g801017 | (1 of 3) K14007 - protein transport protein SEC24 (SEC24) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTAATCTTTACGGTATTGCCATGTGTTGAATCACGTGTTGACCTTTGC |
| Internal bar code: | CGGTCGGTTCATTGTTTGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 360 |
| LEAP-Seq percent confirming: | 38.8889 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCCGTTGTCCATCTTCC |
| Suggested primer 2: | CCACGTATCGGGTGAGTCAG |