Insertion junction: CLIP2.034754_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.034754
Chromosome:chromosome 6
Location:3735757
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278162 PHC3 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 3 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGTGGGGCCGCCGGGGCCGGTGACGCCGGTGGGGCAGCAGTTCTTGGA
Internal bar code:TTCAGCTGTGGGATTTTGGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3257
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGTCCTCTTCCCTCTTAC
Suggested primer 2:GGGGTCAACGTACAGCTTCA