Insertion junction: CLIP2.034760_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.034760
Chromosome:chromosome 15
Location:371777
Confidence (%):80
Locus systematic id Locus common name Defline Feature
lncRNA_TCONS_00212865

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGAATCGAACCTGGCCGGAGCATAACAATTTGGAACCATTTCTCGATT
Internal bar code:AAGCAGCGCTGAAGTTTGTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1511
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:GCTATCACGCTGTGCCATTG
Suggested primer 2:GATGAACACCAGGCTCACGA