| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.034764 |
| Chromosome: | chromosome 9 |
| Location: | 5881258 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g411900 | SHMT3 | (1 of 3) 2.1.2.1 - Glycine hydroxymethyltransferase / Threonine aldolase; Serine hydroxymethyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTTGGTGGTTTTGTGTGGGGACTGAGATCCCCAAATGGGCGCTGTTG |
| Internal bar code: | GGAGCTCCACAGTCAGGCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 234 |
| LEAP-Seq percent confirming: | 69.2308 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCCTACTCCACTTCCAAC |
| Suggested primer 2: | TGGGGACTCGGATAAGCAGA |