Insertion junction: CLIP2.034773_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.034773
Chromosome:chromosome 2
Location:3056149
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095750 ZEP1,ZXE1 (1 of 1) 1.14.13.90 - Zeaxanthin epoxidase / Zea-epoxidase; Putative zeaxanthin epoxidase-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGAGGTTTGATGTGCCAAGCGCTTGCACCGCAGACCAACCCAGGCCGGA
Internal bar code:AGTATGTGACTAAAGAAGAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1067
LEAP-Seq percent confirming:57.5
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:CACGCTTGTCTGCAATACCG
Suggested primer 2:GTGAAGGCGAGATACGGGAG