Insertion junction: CLIP2.034775_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.034775
Chromosome:chromosome 12
Location:7016263
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g560650 (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGTTTGGCGAAATCAACCAACGCCCGAGGCCATGTCCTCAGTCCCTCGC
Internal bar code:TATGCACCAGTCCTCATTATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:5376
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTGCTACAACCGGTACCA
Suggested primer 2:CAACACATGATGAGCGAGCG