| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.034792 |
| Chromosome: | chromosome 4 |
| Location: | 1403247 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214351 | Predicted protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGCTAGGCGATGTGGTCATTCCGGCGGGCGTCACACTGCTTGGGGAG |
| Internal bar code: | GCCTGTTGTTGCTGAGTTCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3474 |
| LEAP-Seq percent confirming: | 95.1219 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCGACAAACCGTACGAGG |
| Suggested primer 2: | CCCGCCTAAGTAGCAGTAGC |