Insertion junction: CLIP2.034841_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.034841
Chromosome:chromosome 9
Location:1577686
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g399000 SDR18 Short-chain dehydrogenase/reductase; (1 of 5) IPR002198//IPR002347//IPR003560//IPR013968 - Short-chain dehydrogenase/reductase SDR // Glucose/ribitol dehydrogenase // 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase // Polyketide synthase, ketoreductase domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGGAGAGCGTGGCGCACAAGGTGCAGGTGAGTGGGGGCGTGGGGGCGT
Internal bar code:CTGACGGGCTCGGGAGGGCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1405
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCACGTAGTCATCCCGGATG
Suggested primer 2:GGGAAACATCACTCAGCGGA