| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.034874 |
| Chromosome: | chromosome 9 |
| Location: | 6285405 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g413950 | EFH6 | EF-hand Calcium binding protein; (1 of 5) PTHR23050:SF90 - CENTRIN-3 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGCGAGCCAACACAGCGCGCGGACGTGTTGCCGCGAGCCCTCACCAGC |
| Internal bar code: | AGGGCGGTACGTACAATTCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5876 |
| LEAP-Seq percent confirming: | 84.2105 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGCAAGAGGTGAGTGGC |
| Suggested primer 2: | TAAGTTCGCAAAGCACACGC |