| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.034879 |
| Chromosome: | chromosome 13 |
| Location: | 1879248 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575550 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAAATACGACGCTGCAAAATGCATCATCATGGATTAGCGACCGCATCG |
| Internal bar code: | ACTGTCTCCTTGTAATTAACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3369 |
| LEAP-Seq percent confirming: | 98.9247 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACTCCCCATGCCTGGATG |
| Suggested primer 2: | CCTGTGCAAAAACGTGGAGG |