| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.034880 |
| Chromosome: | chromosome 11 |
| Location: | 3563055 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477950 | (1 of 1) IPR000104//IPR005502 - Antifreeze protein, type I // ADP-ribosylation/Crystallin J1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAACTCGAGACACCGAAACCCGCCCACCAGAGTTTCCTGTGGTGTTCT |
| Internal bar code: | CGTGCTGTGACGTTGCAATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGGAGACGGTAGCTTGGA |
| Suggested primer 2: | AAGCTCAAGGAGGTGCTGAC |