Insertion junction: CLIP2.034906_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.034906
Chromosome:chromosome 16
Location:7987208
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g691776 (1 of 2) K03145 - transcription elongation factor S-II (TFIIS) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAATAGACAGGTGTAGGTCACGCGCACAAGTCGCGAGCGTGACCACATCT
Internal bar code:ATTTTTACAGGACGCCAACTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:958
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GTCTCAACAGCAGCAGGAGT
Suggested primer 2:TGCAAATCCAAACAGCAGCC