| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.034909 |
| Chromosome: | chromosome 7 |
| Location: | 1191615 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321300 | (1 of 3) PF03703 - Bacterial PH domain (bPH_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACAACAACAATTAGTTATGTGCATAAGTCTCGAGTTCCATGCAAGCCA |
| Internal bar code: | GTTTACTTTATGAAGTGGTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3360 |
| LEAP-Seq percent confirming: | 84.3373 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGAAAGTTGGCCCGGTGT |
| Suggested primer 2: | TGCTGCCCTTAGAGTGTGTG |