Insertion junction: CLIP2.034920_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.034920
Chromosome:chromosome 12
Location:4571100
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g521600 EEP7 (1 of 90) PF03372 - Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos); Endonuclease/exonuclease/phosphatase family protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACACGGCGTGCCGGTAGGGTACAGTGCCGCGCGCGAACACATGAGGGA
Internal bar code:CGCGATCCTGCGAGTATCGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2301
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGCGAAGAGAACCAGCAC
Suggested primer 2:TGTTTTGCTTGGCTGCGTAC