| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.034927 |
| Chromosome: | chromosome 7 |
| Location: | 3009594 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g333535 | (1 of 1) K04856 - voltage-dependent calcium channel T type alpha-1I (CACNA1I) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACCGGCATGCTGGCGCGCGGCACCGCCGCCTGCCTGTACTTCATCACC |
| Internal bar code: | TGCGCGACTGTGTGCCCGCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1539 |
| LEAP-Seq percent confirming: | 98.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATCAGCACCATCCACTT |
| Suggested primer 2: | CACGAGACGTCTATGTCCCG |