| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.034931 |
| Chromosome: | chromosome 5 |
| Location: | 953014 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246900 | (1 of 1) IPR008972//IPR028096 - Cupredoxin // EfeO-type cupredoxin-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCCGTGGGCCAGTCCCTGCATTGGCCCGGGCCCCCAACAATCCATCC |
| Internal bar code: | GACTGTGGCGCCAACCCCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1091 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGGAAGGTGTGAGTGTG |
| Suggested primer 2: | CTGCACGTGTCAAATTGGCA |